FastQCFastQC Report
Thu 26 Oct 2017
Arid1a_1_Ptf1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameArid1a_1_Ptf1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6949357
Sequences flagged as poor quality0
Sequence length27
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAACACTCT2385173.4322168223621268No Hit
CAGACAGGACATGCTACTAAATCGTAT2151783.0963728011095126No Hit
GGTGGCCAGACAGGACATGCTACTAAA1655172.3817599239757No Hit
GGAAGAGCCAGACAGGACATGCTACTA1611822.3193800519961774No Hit
TGTCCCTCAGTTACAGTAAATAAACGA1555822.2387970570514653No Hit
GGAACCAGACAGGACATGCTACTAAAT1539882.215859683133274No Hit
GGTGCCAGACAGGACATGCTACTAAAT1370741.9724702587591916No Hit
GGAAGAGCCCAGACAGGACATGCTACT1143001.6447564861036785No Hit
GGAAGCCAGACAGGACATGCTACTAAA806121.1599922122291315No Hit
ATGATACGGCGACCACCGAACACTCTT766011.1022746420999814No Hit
TTTTCCTCTTGATGCTCAAGAGTTAGT577590.8311416437520767No Hit
GGTGACCAGACAGGACATGCTACTAAA547850.788346317508224No Hit
CTCTTTCCCAGACAGGACATGCTACTA478510.6885673019820394No Hit
GGGGCCAGACAGGACATGCTACTAAAT477730.6874448959810239No Hit
GGAAGACCAGACAGGACATGCTACTAA466480.6712563478894522No Hit
CTCTTTCCAGACAGGACATGCTACTAA455830.655931189029431No Hit
GGAAGAGCGTCCAGACAGGACATGCTA446410.6423759780940884No Hit
GGTGTCCAGACAGGACATGCTACTAAA439230.6320440869565342No Hit
GGAAGAGCGCCAGACAGGACATGCTAC425150.6117832196561495No Hit
GGAAGAGCGTCCCAGACAGGACATGCT401450.5776793450099053No Hit
AGTTACAGCAGGAGAAAAATTACCTGA370820.5336033247392529No Hit
AATGATACGGCGACCACCGAACACTCC350820.5048236836875699No Hit
GGTGGTCCAGACAGGACATGCTACTAA340030.48929706734018696No Hit
GGGTCCAGACAGGACATGCTACTAAAT293840.4228304863313253No Hit
AAAACCGGCTATTTACTGATGTCATTT279200.4017637890814934No Hit
GGACCAGACAGGACATGCTACTAAATC277860.3998355531310307No Hit
GGTGGTACCAGACAGGACATGCTACTA265900.38262532778212427No Hit
AACACTCTTTCCCAGACAGGACATGCT237470.341715068027157No Hit
GGTGGTCCCAGACAGGACATGCTACTA228010.32810229780971106No Hit
GGGTACCAGACAGGACATGCTACTAAA224650.3232673181130283No Hit
GGTGGGCCAGACAGGACATGCTACTAA221300.3184467282368714No Hit
GGTGGTGTCCAGACAGGACATGCTACT204260.2939264740608376No Hit
TAGCCGTTCATACATTAGACTCAACAA193610.27860131520081644No Hit
CTTCCTCCGCTGCTTTTCATTAGGCAC192770.27739257027664577No Hit
GGTGGACCAGACAGGACATGCTACTAA177020.25472860294844546No Hit
GGTGTGCCAGACAGGACATGCTACTAA171720.24710199806974947No Hit
GGTGGTCGCCAGACAGGACATGCTACT169950.24455499983667556No Hit
TCAGCTGGCGGATCCCAGACAGGACAT161940.23302875359547653No Hit
GGAGGCCAGACAGGACATGCTACTAAA160820.2314170936965823No Hit
GGCCAGACAGGACATGCTACTAAATCG155260.22341635348421446No Hit
GTACGGCGACCACCGAACACTCTTTCC149580.2152429354255365No Hit
GGGCGCCAGACAGGACATGCTACTAAA148640.21389029229610743No Hit
GGGGGCCAGACAGGACATGCTACTAAA148590.2138183431934782No Hit
GTGGCCAGACAGGACATGCTACTAAAT148350.213472987500858No Hit
GTGGTCCAGACAGGACATGCTACTAAA143840.2069831784437035No Hit
GGGTGCCAGACAGGACATGCTACTAAA143150.20599028082742044No Hit
GGGCCGCCAGACAGGACATGCTACTAA139300.20045019992497148No Hit
GGGGTCCAGACAGGACATGCTACTAAA139080.20013362387340297No Hit
GGGTGACCAGACAGGACATGCTACTAA138660.1995292514113176No Hit
GGTGGTCGCCCAGACAGGACATGCTAC138070.198680252000293No Hit
GGTGCCGCCAGACAGGACATGCTACTA134650.1937589333804552No Hit
GGTGGATCCAGACAGGACATGCTACTA132680.19092413873686445No Hit
GGTACCAGACAGGACATGCTACTAAAT131940.18985929201795218No Hit
AACACTCTTTCCAGACAGGACATGCTA131020.18853542852957475No Hit
GGTGGGTCACCAGACAGGACATGCTAC129150.1858445320912424No Hit
GTGGACCAGACAGGACATGCTACTAAA124920.17975763800881145No Hit
GGTGGGTACCAGACAGGACATGCTACT124550.1792252146493553No Hit
GGTGGCCGCCAGACAGGACATGCTACT123350.17749843618625435No Hit
GGGCCAGACAGGACATGCTACTAAATC120010.17269223613062332No Hit
GGGGACCAGACAGGACATGCTACTAAA120000.17267784631009747No Hit
GGTGGTGGCCAGACAGGACATGCTACT119970.17263467684851994No Hit
GACCACCGAACACTCTTTCCAGACAGG119710.17226054151484807No Hit
GGTGCGCCAGACAGGACATGCTACTAA118220.17011645825649768No Hit
GGTCCAGACAGGACATGCTACTAAATC118170.17004450915386848No Hit
GGGGCCGCCAGACAGGACATGCTACTA117390.16892210315285286No Hit
GGGGCGTACCAGACAGGACATGCTACT115680.16646144384293396No Hit
GGTTGCCAGACAGGACATGCTACTAAA114710.16506563125192733No Hit
GGGTCGCCAGACAGGACATGCTACTAA113600.16346836117355892No Hit
ACCACCGAACACTCTTTCCCAGACAGG113150.16282081924989608No Hit
GTCACCAGACAGGACATGCTACTAAAT112290.1615832946846737No Hit
GTGCGCCAGACAGGACATGCTACTAAA110570.15910824555422898No Hit
GTGTTCCAGACAGGACATGCTACTAAA109810.15801461919426502No Hit
GGTATCCAGACAGGACATGCTACTAAA109050.15692099283430108No Hit
ACCTCTTCCAGACAGGACATGCTACTA107820.1551510449096226No Hit
GTGGGCCAGACAGGACATGCTACTAAA105130.1512801831881712No Hit
GGTTCCAGACAGGACATGCTACTAAAT105040.15115067480343866No Hit
TCATTCCAGACAGGACATGCTACTAAA104870.15090604785449935No Hit
CCGACACTCTTTCCCAGACAGGACATG102920.14810003285196027No Hit
GGGTCACCAGACAGGACATGCTACTAA102780.14789857536459847No Hit
GGGCCGACCAGACAGGACATGCTACTA101720.1463732543888593No Hit
GGGGATCCAGACAGGACATGCTACTAA100810.1450637807210077No Hit
GGTGTCGGCCAGACAGGACATGCTACT100260.14427234059208643No Hit
GGGGTCGGCCAGACAGGACATGCTACT98820.14220020643636525No Hit
GGTGTACCAGACAGGACATGCTACTAA97710.14060293635799687No Hit
GGGACCAGACAGGACATGCTACTAAAT97150.13979710640854973No Hit
GGAAGAGCGTCGCCAGACAGGACATGC97120.13975393694697222No Hit
GTGGTATCCAGACAGGACATGCTACTA96430.13876103933068915No Hit
GTGGCGCCAGACAGGACATGCTACTAA96240.13848763274069817No Hit
GGTGCGTCCAGACAGGACATGCTACTA95410.13729327763705332No Hit
AGACAGGACATGCTACTAAATCGTATG94500.13598380396920176No Hit
CGGCCGAACACTCTTTCCCAGACAGGA94030.13530748240448723No Hit
GGTGGTCGGTTCACCAGACAGGACATG92600.13324973806929188No Hit
ACCGAACACTCTTTCCCAGACAGGACA92410.13297633147930088No Hit
GGCCGCCAGACAGGACATGCTACTAAA91970.13234317937616386No Hit
ACACTCTTCCCAGACAGGACATGCTAC91030.13099053624673476No Hit
GGTGGCGTACACCAGACAGGACATGCT90870.13076029911832132No Hit
GGTGGTCGGCCAGACAGGACATGCTAC90810.13067396019516628No Hit
GGTGGGCGTATCACCAGACAGGACATG90680.13048689252833032No Hit
CCACACCGAACACTCTTTCCAGACAGG89810.1292349781425821No Hit
TCACCGAACACTCTTTCCAGACAGGAC87310.12563752301112174No Hit
GGAAGCGAACCAGACAGGACATGCTAC87160.12542167570323412No Hit
GGTGGTCGCCGCCAGACAGGACATGCT86820.12493242180535552No Hit
GGTGCGGCCAGACAGGACATGCTACTA85320.1227739487264793No Hit
ACTCTTTCCCAGACAGGACATGCTACT84710.12189616967440296No Hit
GTGTCCAGACAGGACATGCTACTAAAT84020.12090327205811992No Hit
ATTCAGCTCATGTATCTTTGCTCATGC83770.12054352654497387No Hit
GGCGGCCAGACAGGACATGCTACTAAA83640.12035645887813794No Hit
GAAGACGTCGTAGAGCCAGACAGGACA83600.12029889959603457No Hit
GTGCACCAGACAGGACATGCTACTAAA83550.12022695049340534No Hit
GTGTCGTACACCAGACAGGACATGCTA83510.12016939121130199No Hit
GGTGGTCGGTACCAGACAGGACATGCT82350.1185001720303044No Hit
ACGGCACCACCGAACACTCTTTCCCAG82200.11828432472241676No Hit
GGTGGTCGCCCCAGACAGGACATGCTA82150.11821237561978756No Hit
ATTGTTGTTTTACCCAGACAGGACATG81920.11788140974769319No Hit
GGTGCGACCAGACAGGACATGCTACTA81890.11783824028611567No Hit
GGTGGGTCCAGACAGGACATGCTACTA80950.11648559715668658No Hit
CTGCAGCGGAGCAGAACGCTGTGAAAG80130.11530563187356759No Hit
GGGGGCACCAGACAGGACATGCTACTA79720.11471564923200808No Hit
GGTGGTCGGACCAGACAGGACATGCTA78910.11355007376941492No Hit
CATCTTCCCAGACAGGACATGCTACTA78760.11333422646152731No Hit
GGTGGACCCTCTACAATACCCTCTCCA78140.11244205758892513No Hit
CCAGACAGGACATGCTACTAAATCGTA77870.1120535324347274No Hit
GGTGGTCGTACCAGACAGGACATGCTA77000.1108016180489792No Hit
ACATGAGGGTAAATAATTTTGACTTCA76650.11029797433057476No Hit
GGTGGGCACCAGACAGGACATGCTACT76620.11025480486899722No Hit
GGTCGTATCCAGACAGGACATGCTACT76340.10985188989427368No Hit
GGTGTCGCCAGACAGGACATGCTACTA76260.10973677133006693No Hit
GGGGCGGCCAGACAGGACATGCTACTA75750.10900289048324903No Hit
CCCCGAACACTCTTTCCCAGACAGGAC75050.10799560304644011No Hit
AAACCAGACAGGACATGCTACTAAATC74860.10772219645644913No Hit
GGAGCCAGACAGGACATGCTACTAAAT73910.10635516350649421No Hit
GGGGGACCAGACAGGACATGCTACTAA73310.1054917742749437No Hit
GGTGGCGACCAGACAGGACATGCTACT73210.1053478760696853No Hit
GGGTTCCAGACAGGACATGCTACTAAA73170.10529031678758194No Hit
GAATATCCAGACAGGACATGCTACTAA72970.10500252037706509No Hit
GGAAGAGGGCCAGACAGGACATGCTAC72340.1040959616839371No Hit
AATGATACACTCTTTCCCAGACAGGAC71810.10333330119606748No Hit
CTCTTCCCAGACAGGACATGCTACTAA71360.10268575927240463No Hit
GTGGTCCGTTCACCAGACAGGACATGC71280.1025706407081979No Hit
CCCGAACATCTTTCCAGACAGGACATG70950.10209577663084513No Hit
GGTGTCACCAGACAGGACATGCTACTA70870.10198065806663839No Hit
GTGCGCCGCCAGACAGGACATGCTACT70750.1018079802203283No Hit
GGGTGATCCAGACAGGACATGCTACTA70670.10169286165612158No Hit
GTGGCTCCAGACAGGACATGCTACTAA70620.10162091255349236No Hit
GGTGGCCCAGACAGGACATGCTACTAA70230.10105970955298453No Hit
GGCACCAGACAGGACATGCTACTAAAT70160.10095898080930364No Hit
GGGCGACCAGACAGGACATGCTACTAA69860.1005272861935284No Hit
GGGATCCAGACAGGACATGCTACTAAA69730.10034021852669246No Hit
GGGCACCAGACAGGACATGCTACTAAA69710.10031143888564079No Hit
GTGACCAGACAGGACATGCTACTAAAT69570.10010998139827901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGG250.003379123821.00253521
ATCAGGG405.4112497E-621.00253521
TAAACGC850.021.00253521
ACCTGAA250.003379123821.00253521
TCGTCTG250.003379123821.00253521
ACTGTAT250.003379123821.00253521
GCAGCAT250.003379123821.00253521
TATACGA250.003379123821.00253521
ATCGTAG303.9331822E-421.00253321
CTCTCAA303.9331822E-421.00253321
GGTTAGT456.3874904E-721.00253321
ATCGCAT900.021.00253321
TCAACGA601.0713848E-921.00253321
TCAAAGC751.8189894E-1221.00253321
CCCCTGT1900.021.0020818
ATCTATA558.991265E-921.0020815
TAAAGGC3100.021.0020815
CTTCATT250.003379546821.0020815
CACATTT558.991265E-921.0020818
GAGTATT250.003379546821.0020815
Produced by FastQC (version 0.11.5)